After some great feedback from our users, we’ve made feature updates and software improvements with the latest release of v18.104.22.168. The changes from v22.214.171.124 to v126.96.36.199 include the following:
We made some improvements to the noise rejection algorithm so that smoother curves are observed at the end of each run.
Improved Wi-Fi Performance
Previously, we noticed that the software would constantly scan for Wi-Fi networks when the browser application was open. This contributed to some connection issues and might have caused a delayed response in accessing the local network. We have since mitigated this - the software now scans for Wi-Fi network settings only when you open the Wi-Fi Settings page.
Adjustable Baseline Cycles
We’ve incorporated an Amplification Settings option on the results screen. Once you access this, you can opt for a manual adjustment of the baseline cycles post-run.
For those of us who are not biologists by trade, baseline is the background signal level prior to any significant product amplification. The software's baseline subtraction algorithm aligns the baselines of each curve, as you can see in the picture above. Our algorithms automatically detect the best baseline in most cases, but starting with v188.8.131.52 you can override the cycles which should be considered for baseline analysis. This might be necessary in cases where you have a very early amplification curve.
Cq Calling Methods
We’ve given you the option to select which Cq calling method you’d like to use for each analysis. The default and recommended setting is the Cy0 method, which is based on max first derivative of the curve. However, feel free to tinker with the cpD2 method (max second derivative of the curve) since the characteristics of the data itself can affect how each Cq method perform.
If you’ve already updated the software, you probably noticed the design layout for well selection has changed. Now you can select and deselect multiple wells at one time! (Yes, finally right? :)) There are a couple options you can go about doing this. You can drag to select all adjacent wells simultaneously or select noncontiguous wells. To view data from noncontiguous wells, click on each well while holding down the Command key (for Mac users) or the Control key (for PC users).
Sample Name Input
We realize that it’s a hassle to remember all the different samples you’ve run so we incorporated the sample naming feature into the result page. Once your run completes, simply click under the Sample Name column, type in your respective sample names, and everything will be automatically saved.
If you have any specific questions about a software feature, comment below and we’ll get back to you shortly :)